Structures and mechanisms of glycosyl hydrolases
نویسندگان
چکیده
منابع مشابه
Bioprospecting metagenomes: glycosyl hydrolases for converting biomass
Throughout immeasurable time, microorganisms evolved and accumulated remarkable physiological and functional heterogeneity, and now constitute the major reserve for genetic diversity on earth. Using metagenomics, namely genetic material recovered directly from environmental samples, this biogenetic diversification can be accessed without the need to cultivate cells. Accordingly, microbial commu...
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This study aimed to concentrate glycosyl hydrolases produced by Trichoderma harzianum P49P11 by ethanol precipitation. The variables tested besides ethanol concentration were temperature and pH. The precipitation with 90% (v/v) ethanol at pH 5.0 recovered more than 98% of the xylanase activity, regardless of the temperature (5.0, 15.0, or 25.0 °C). The maximum recovery of cellulase activity as ...
متن کاملDetermination of the catalytic base in family 48 glycosyl hydrolases.
The catalytic base in family 48 glycosyl hydrolases has not been previously established experimentally. Based on structural and modeling data published to date, we used site-directed mutagenesis and azide rescue activity assays to show definitively that the catalytic base in Thermobifida fusca Cel48A is aspartic acid 225. Of the tested mutants, only Cel48A with the D225E mutation retained parti...
متن کاملNomenclature for sugar-binding subsites in glycosyl hydrolases.
The huge structural diversity of polysaccharides leads to their central roles in food storage and utilization, structure, cell–cell signalling, cell-wall expansion and turnover and viral invasion. Glycosyl hydrolases, enzymes hydrolysing the glycosidic bond in di-, oligoand poly-saccharides, are found in all living organisms. The first X-ray structural determination of an enzyme was of a glycos...
متن کاملExpression, Characterization and Synergistic Interactions of Myxobacter Sp. AL-1 Cel9 and Cel48 Glycosyl Hydrolases
The soil microorganism Myxobacter Sp. AL-1 regulates in a differential manner the production of five extracellular cellulases during its life cycle. The nucleotide sequence of a cel9-cel48 cluster from the genome of this microorganism was recently obtained. Cel48 was expressed in Escherichia coli to generate a His(6)-Cel48 protein and the biochemical properties of the pure protein were determin...
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ژورنال
عنوان ژورنال: Structure
سال: 1995
ISSN: 0969-2126
DOI: 10.1016/s0969-2126(01)00220-9